The purpose of this study is to evaluate the effects of prebiotic, probiotic and synbiotic supplementation on liver histopathology and TLR-4, NFκB and TNF-α gene expression involved in the inflammatory cascade and pathogenesis of experimental nonalcoholic fatty liver disease (NAFLD).
Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (60 days). On Day 30 of hypercholesterolemic conditions, rats were subdivided in five groups: negative control (NC), positive control (PC), prebiotic (PRE), probiotic (PRO) and synbiotic (SYN). All rats were sacrificed on Day 60. Liver tissue was used to verify histopathological changes and gene expression. Gene expression of TLR-4, TNF-α and NFκB was evaluated in liver tissue using RT-qPCR.
Histopathological analysis: PC showed more changes than NC, and PRE and SYN showed fewer alterations than PC. Gene expression analysis: PRE showed higher TLR-4, and NFκB and TNF-α compared to PC. Also, PRE showed higher TLR-4 when compared to PRO and SYN. SYN group revealed higher TLR-4 and NFκB expressions compared to PC. PRO group also showed higher NFκB expression compared to PC.
NAFLD is a significant health concern, and it found that prebiotic, probiotic and synbiotic supplementation could have positive effects as a nonpharmacological approach to control this disease.
This study was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), process number 2011/50289-1 and 2017/18524-7.
The authors are also grateful to Farmoquímica – FQM São Paulo/Brazil for providing the prebiotic, probiotic and synbiotic supplements.
Carreira dos Santos, A., Ribeiro, D., Ferreira, J., Aguiar, O., Waitzberg, D. and Alves, C. (2019), "Inflammatory gene expression analysis after prebiotic, probiotic and synbiotic supplementation in experimental nonalcoholic fatty liver disease", Nutrition & Food Science, Vol. 49 No. 1, pp. 75-84. https://doi.org/10.1108/NFS-04-2018-0119Download as .RIS
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