Provitamin A cassava hydrolysate with Lactobacillus rhamnosus GG regulates weight gain, lipids and postprandial glycemia in wistar rats and humans
ISSN: 0034-6659
Article publication date: 28 December 2021
Issue publication date: 7 February 2022
Abstract
Purpose
This study aims to evaluate the effect of probiotic provitamin A cassava hydrolysate with Lactobacillus rhamnosus GG (hLGG) on weight and lipid profile of Wistar rats and its glycemic index using Wistar rats and human subjects.
Design/methodology/approach
Adult male Wistar rats (n = 40, 120–150 g) were orally administered provitamin A cassava hydrolysate with 1 × 1010, 2 × 1010 and 4 × 1010 CFU/g encapsulated or CFU/mL free Lactobacillus rhamnosus GG for 30 days, during which weight and lipid profile of rats were monitored. Blood glucose levels of rats and human subjects were also measured in Oral Glucose Tolerance Test to determine the Glycemic indices of hLGG.
Findings
Rats administered the highest doses of free or encapsulated hLGG [(4 × 1010 CFU) (PHE4 and PHF4, respectively)] had the lowest (18.2 ± 0.7 and 8.0 ± 0.6%, respectively, p < 0.001) percentage body weight gain compared to control (40 ± 0.6%). Lowest cholesterol and triglyceride (42.4 ± 0.5 and 44.4 ± 0.7 g/dL, p < 0.001, respectively) were observed in rats administered PHE4, with the lowest plasma glucose concentrations in PHE4 and PHF4 groups (43 ± 1 and 49 ± 0.7 g/dL, p < 0.001, respectively). Oral Glucose Tolerance Test for rats and human subjects showed lower peak blood glucose levels and glycemic indices in hLGG groups compared to controls in a dose-dependent manner.
Originality/value
Consumption of soft drinks, which supply non-nutritive energy, may lead to degenerative metabolic disorders such as obesity and diabetes. Beverages with probiotics such as Lactobacillus rhamnosus GG, on the other hand, offer a positive weight management approach. Development of non-dairy beverages such as provitamin A cassava hLGG is ongoing. Provitamin A cassava hLGG showed its ability to control weight gain, blood glucose levels and serum lipids. Thus, the beverage can be consumed as a healthy alternative to soft drinks and for weight management.
Keywords
Acknowledgements
The authors are thankful to the Cassava Breeding Unit, International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, for providing the PAC samples used in this research.
Gratitude also goes to Professor Victoria Adetunji, of the Department of Veterinary and Public Health, University of Ibadan, Nigeria, for providing the Lactobacillus rhamnosus GG used in this study.
Conflict of interest: The authors confirm that there are no conflicts of interest regarding the work described in this manuscript.
Funding sources: This research did not receive any significant grant from funding agencies in the public, private or not-for-profit sectors.
Citation
Oguntoye, M., Ezekiel, O. and Oridupa, O. (2022), "Provitamin A cassava hydrolysate with
Publisher
:Emerald Publishing Limited
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