To read the full version of this content please select one of the options below:

Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection

Sharipah Nadzirah (Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP) , Kangar, Malaysia)
Uda Hashim (Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP) , Kangar, Malaysia)

Microelectronics International

ISSN: 1356-5362

Article publication date: 2 May 2017

Downloads
123

Abstract

Purpose

The purpose of this study is to fabricate a transducer-based TiO2 interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for Escherichia. coli O157:H7 DNA detection.

Design/methodology/approach

Sol-gel was used to synthesize TiO2 nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.

Findings

TiO2 nanoparticles IDEs with 16-um gap size is able to detect DNA of E. coli in a dry state.

Originality/value

This paper describes IDEs for dry-state detection of E. coli O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.

Keywords

Acknowledgements

The author would like to thank all staff members of the Institute of Nano Electronic Engineering at Universiti Malaysia Perlis (UniMAP) for their technical advice and contributions, both directly and indirectly.

Citation

Nadzirah, S. and Hashim, U. (2017), "Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection", Microelectronics International, Vol. 34 No. 2, pp. 99-107. https://doi.org/10.1108/MI-08-2016-0054

Publisher

:

Emerald Publishing Limited

Copyright © 2017, Emerald Publishing Limited