Production of efficient enzymes for flax retting by solid state fermentation with Aspergillus niger
International Journal of Clothing Science and Technology
ISSN: 0955-6222
Article publication date: 27 May 2014
Abstract
Purpose
The purpose of this paper is to study the optimum culture condition of flax-retting enzyme, and applying the enzyme liquors to retting. In addition to research the structure of flax stem in enzyme retting using scanning electron microscopy (SEM).
Design/methodology/approach
The influence of cultural parameters such as moisture level, temperature, incubation time and inoculum size were evaluated with respect to polygalacturonase and xylanase yield. The structure of flax stem was revealed by SEM.
Findings
The flax was retted by dilute enzyme liquors (polygalacturonase/25 U/ml, xylanase/1 U/ml), and 24-h retting period was obtained. The SEM studies revealed the removal of a large amount of non-cellulosic gummy material of flax stem in enzyme retting.
Originality/value
First, flax-retting enzyme containing higher-yield polygalacturonase, lower-yield xylanase and no cellulose was yielded by SSF. Second, the composition of enzyme could meet flax retting and no damage cellulosic fibre. Finally, only low-cost wheat bran, citrus peel powder and mineral salt were employed in substrate, which could reduce the overall cost of enzyme production and flax retting.
Keywords
Acknowledgements
The authors express thanks to Nature Science Foundation of Heilongjiang, China, for the financial assistance. The authors also wish to thank Key Laboratory of Processing Agricultural Products of Heilongjiang Province, Qiqihar University.
Citation
Yinghua, T., Xiaolan, L., Xiqun, Z. and Lu, W. (2014), "Production of efficient enzymes for flax retting by solid state fermentation with
Publisher
:Emerald Group Publishing Limited
Copyright © 2014, Emerald Group Publishing Limited