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ISFET structures with chemically modified membrane for bovine serum albumin detection

Piotr Firek (Institute of Microelectronics and Optoelectronics, Warsaw University of Technology, Warsaw, Poland)
Michal Cichomski (Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lódz, Poland)
Michal Waskiewicz (Institute of Microelectronics and Optoelectronics, Warsaw University of Technology, Warsaw, Poland)
Ireneusz Piwoński (Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lódz, Poland)
Aneta Kisielewska (Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lódz, Poland)

Circuit World

ISSN: 0305-6120

Article publication date: 11 January 2018

Issue publication date: 12 February 2018

Abstract

Purpose

The purpose of this paper is to present possibility of fast and certain identification of bovine serum albumin (BSA) by means of ion-sensitive field effect transistor (ISFET) structures. Because BSA can cause allergic reactions in humans, it is one of reasons for development of sensitive sensors to detect residual BSA. BSA is commonly used in biochemistry and molecular biology in laboratory experiments. Therefore, to better understand the mechanism of signal transduction in simulated biological environment and to elucidate the role of adsorption of biomolecules in the generation of a signal at the interface with biological systems, the measurements of ISFET current response in the presence of BSA as a reference protein molecule were performed.

Design/methodology/approach

To fabricate transistors, silicon technology was used. The ISFET structures were coupled to specially designed double-side printed circuit board holder. After modification of the field effect transistor (FET) device with 3-aminopropyltriethoxysilane (APTES), a sensor with high sensitivity toward reference biomolecules was obtained. The current–voltage (I-V) characteristics of structures with and without gate modification were measured. Keithley SMU 236/237/238 measurement set was used. Deionized water solution and 0.05 per cent BSA were used.

Findings

In this research, a method of preparation of a biosensor based on a FET was developed. Sensitivity of APTES-modified FET device toward BSA as a biomolecule was investigated. I-V relationships of FET devices (with and without modification), being the effect of the interactions with the solution containing 0.05 per cent BSA, were measured and compared to the measurements performed for solutions without BSA.

Originality value

Compared to SiO2-containing ISFETs without modification or other different dielectrics, the application of APTES as the part of the membrane induced significant increase in their sensitivity to BSA.

Keywords

Acknowledgements

This work was supported by The National Science Centre (Grant no N N515 498140, UMO-2011/01/D/ST7/00640).

Citation

Firek, P., Cichomski, M., Waskiewicz, M., Piwoński, I. and Kisielewska, A. (2018), "ISFET structures with chemically modified membrane for bovine serum albumin detection", Circuit World, Vol. 44 No. 1, pp. 45-50. https://doi.org/10.1108/CW-10-2017-0061

Publisher

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Emerald Publishing Limited

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