Biotypes and serotypes of Campylobacter spp. isolated from broilers in Trinidad

Shelly Rodrigo (School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St Augustine, Trinidad and Tobago)
Abiodun Adesiyun (School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St Augustine, Trinidad and Tobago)
Zinora Asgarali (School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St Augustine, Trinidad and Tobago)

British Food Journal

ISSN: 0007-070X

Publication date: 1 December 2005

Abstract

Purpose

This paper sets out to investigate the biotypes of Campylobacter jejuni and C. coli and the serotypes of C. jejuni present in broilers from selected small retail processors in six counties in Trinidad.

Design/methodology/approach

Samples were plated on blood‐free Campylobacter CCDA, incubated at 42°C in 8‐10 per cent CO2 in a CO2 incubator and isolates were biotyped using the Lior scheme and serotyped using 25 Penner heat‐stable antisera by the passive hemagglutination method.

Findings

Amongst the 743 C. jejuni isolates biotyped, 85.3 per cent and 14.7 per cent belonged to biotype I and II respectively compared with C. coli isolates where 84.3 per cent were grouped as biotype I and 15.7 per cent were biotype II amongst a total of 681 isolates. The difference in frequency of both biotypes amongst C. jejuni and C. coli was statistically significant (p<0.05; χ2). Of the 52 isolates of C. jejuni, 48 (92.3 per cent) were typable. The predominant serotype Penner HS O:31 accounted for 19.2 per cent of the isolates tested, while Penner HS O:2, the predominant serotype recovered from clinical cases world‐wide, accounted for 5.8 per cent. Originality/value – The biotypes detected in Campylobacter isolates from chickens are similar with those earlier isolated from other livestock in Trinidad. However, this is the first documentation of serotypes of C. jejuni in this country. Serotypes O:31 and O:2 have been isolated from clinical cases world‐wide and may have clinical implications in Trinidad. It is, however, pertinent to mention that until C. jejuni is routinely serotyped and reporting of human clinical cases of campylobacteriosis in the country is improved, the clinical significance of C. jejuni serotypes cannot be fully appreciated.

Keywords

Citation

Rodrigo, S., Adesiyun, A. and Asgarali, Z. (2005), "Biotypes and serotypes of Campylobacter spp. isolated from broilers in Trinidad", British Food Journal, Vol. 107 No. 11, pp. 884-890. https://doi.org/10.1108/00070700510629814

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Publisher

:

Emerald Group Publishing Limited

Copyright © 2005, Emerald Group Publishing Limited

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