To read the full version of this content please select one of the options below:

Validation of a PCR method for Campylobacter detection on poultry packs

TeckLok Wong (Food Safety Group, Institute of Environmental Science and Research Ltd, Christchurch Science Centre, Ilam, Christchurch, New Zealand)
Megan Louise Devane (Water Group, Institute of Environmental Science and Research Ltd, Christchurch Science Centre, Ilam, Christchurch, New Zealand)
John Andrew Hudson (Food Safety Group, Institute of Environmental Science and Research Ltd, Christchurch Science Centre, Ilam, Christchurch, New Zealand)
Paula Scholes (Water Group, Institute of Environmental Science and Research Ltd, Christchurch Science Centre, Ilam, Christchurch, New Zealand)
Marion Grace Savill (Water Group, Institute of Environmental Science and Research Ltd, Christchurch Science Centre, Ilam, Christchurch, New Zealand)
John D. Klena (School of Molecular Sciences, Washington State University, Washington, USA)

British Food Journal

ISSN: 0007-070X

Article publication date: 1 September 2004

Abstract

This study describes a multiplex polymerase chain reaction (PCR) detection system combined with enrichment growth conditions for simultaneous detection and identification of C. jejuni, C. coli and thermotolerant Campylobacter in poultry pack rinses. The PCR primers were tested against a range of Campylobacter and non‐Campylobacter species, and PCR products were only amplified from target organisms. The sensitivity of the method was similar to that obtained by conventional plating procedures, but when used in combination with the MPN method of enumeration could detect levels down to 6 MPN/100 ml of rinse. The validation of 50 samples of chicken pack rinses demonstrated the versatility of this approach in microbiological surveys to yield data for risk assessments.

Keywords

Citation

Wong, T., Devane, M.L., Hudson, J.A., Scholes, P., Grace Savill, M. and Klena, J.D. (2004), "Validation of a PCR method for Campylobacter detection on poultry packs", British Food Journal, Vol. 106 No. 9, pp. 642-650. https://doi.org/10.1108/00070700410558175

Publisher

:

Emerald Group Publishing Limited

Copyright © 2004, Emerald Group Publishing Limited